b'TRANSFER EFFICACY STUDYTHIRD PARTY DATA AIONX makes no claims herein. AIONX makes no claims herein.See bottom for full legal disclaimer. See bottom for full legal disclaimer.REDUCTION OF BACTERIA ON EVEN NON-COVERED SURFACES1.Clinical StudiesDirect, Continuous Reduction of BioburdenAfter the introduction of cleanSURFACES, the abundance of a large number of bacterial species was found to be eliminated or significantly reduced throughout the unit.Not only did cleanSURFACES keep covered surfaces continuously clean, but many key surfaces that wereEsolen LM, Thakur L, Layon A, et. al. (2018).ICU-based study on gen 1 cleanSURFACES demonstrating continuous reduction of bacteria on the not coveredby the product were also found to be significantly cleaner. The below chart sets forth summaries of the bacterial species with aThe Efficacy of a Self-disinfecting Bedrailproducts compared to similar uncovered surfaces. Over a multi-week period, CleanSurfaces placed on change in mean abundance (p-value 0.1) on each of the non-covered surfaces sampled.Cover in an Intensive Care Unit. Am J Infectbed rails demonstrated continuous 80% reduction of bacteria (including a significant, continuous Control, 4(46), 417-19.reduction of key pathogens), as well as a nearly 5x increase in the surfaces free of bacteria. Total # of species withPatel R, MD, et al. (2019). CurrentlyTriple-blind, ICU-based study on cleanSURFACES demonstrating 45 days of continuous reduction of change in meanSpecies eliminatedSpecies significantly Total species unpublished manuscript, Easton Hospital.bacteria on the products compared to similar uncovered surfaces. As much as a seven-fold increase in reduced but not eliminated eliminated or reducedsurfaces rendered non-infective was observed, depending on the infective threshold assumed. abundanceEfficacy Transfers to Other SurfacesKeyboard, patient room182162(89%)14(8%)176(97%)Lamendella R, PhD, Wright JR, Shope AJ, MD.Cutting-edge study exploring the impact on the contamination transmission network of strategic Mouse, patient room270236(87%)27(10%)263(97%)(2019). Currently unpublished report,placement of cleanSURFACES in an ICU. This study found that use of the CleanSurfaces on several Contamination Source Identification, Inc. surfaces with high impact in the contamination transmission network significantly reduced bacteria IV pump, patient room196176(90%)14(7%)190(97%)(including in spore form) on many high touch surfaces NOT covered with the products. For example, Supply scanner, patient room253214(85%)28(11%)242(96%)89% of bacterial species were eliminated on the nearby but uncovered WOW keyboards. Many key Nurse ID badge114101(89%)8(7%)109(96%)bacterial species, including C. diff, were among those eliminated from all samples.Keyboard, nurse station172130(76%)34(20%)164(95%)2.Lab StudiesPhone, nurse station14484(58%)35(24%)119(83%)BacteriaMouse, nurse station4918(37%)4(8%)22(45%)Agile Sciences, Inc. (2014). UnpublishedTests on gen 1 cleanSURFACES technology demonstrate 6 log 10reduction of E. coli in 5 minutes and Cellphone330(0%)13(39%)13(39%)report.up to 5 log 10reduction of MRSA in 10 minutes. More recent tests are also included for your review. Glucometer, nurse station3913(33%)2(5%)15(38%) Stethoscope490(0%)15(31%)15(31%)Viruses Chair rails, nurse station9117(19%)1(1%)18(20%)Medenhall M. (2019). Unpublished report,Tests on the cleanSURFACES technology demonstrated the reduction of H1N1 virus of 1.7 log 10and Utah State University.4.7 log 10at 10 and 30 minutes, respectively, and of murine norovirus of 1.2 log 10and 4.5 log 10at 10 and 30 minutes, respectively. IMPACT ON CLINICALLY RELEVANTC. diff SporesBACTERIAL SPECIES HOW THE ANALYSIS WAS DONECadnum J, Livingston S,. (2018). UnpublishedTests on gen 1 cleanSURFACES technology demonstrated up to 1.46 log 10reduction of C. diff data, Curtis Donskey Research Services.spores. Researchers from Contamination Source Identification, LLC Many clinically relevant bacterial species were among those eliminated or significantly reduced during the post- (Huntingdon, PA), designed the study, analyzed the samples, and intervention period.The below chart shows percentperformed the statistical calculations. Metagenomic analysis of3. Safety Studiesreduction in abundance across all samples of several keythe samples was done using 16S rRNA amplicon sequencingBiocompatibility Study techniques coupled with a proprietary bioinformatic data bacterial species. (p 0.05) pipeline. Results of this analysis revealed the presence ofSamberg et al., J Mater Sci: Mater Med (OctStudy demonstrating good biocompatibility of electrically-stimulated ionic silver with three 2012), DOI 10.1007/s10856-012-4838-5.mammalian cell lines (keratinocytes, osteoblasts, and fibroblasts). particular bacterial taxa in the samples as well as each taxons % Reductionabundance.Bacterial Species(All Samples)EPA-Required Animal StudiesProduct Safety Labs. (2016). UnpublishedThe following studies were performed by a third-party lab as part of the EPA registration process: (i) C. diff100reports.dermal sensitization test (guinea pigs); (ii) acute oral toxicity (rats); (iii) acute dermal toxicity (rats); (iv) Enterococcus spp.79primary eye irritation (rabbits); and (v) primary skin irritation (rabbits). Bacteroides fragilis100cleanSURFACES were created by Staphyloccocus aureus45 Aionx Antimicrobial Technologies, Inc.4. Current, Relevant Research Acinetobacter baumannii100515 Rear West Chocolate Avenue Pathogenic Streptococcus spp.45Hershey, PA 15044Better Cleaning of Key Surfaces Reduces Contamination Throughout Facility Morganella morganii100(888) 512-4669 | firstname.lastname@example.orgReynolds, et al. (2019, in press). Am J InfectPublished study describes the impact on environmental contamination in an entire healthcare clinic of aionx.comControl.manual disinfection of targeted high touch surfaces. One-time disinfection of select surfaces briefly Mycobacterium abscessus100reduced surface contamination throughout the clinic by 94%. Reduced Environmental Contamination Correlates with Reduced Infection Rate Rutala, et al. (2018). Infect Control HospThis is the first study to describe the full pathway whereby enhanced disinfection decreases microbial Epidemiol.contamination, which in turn reduces patient colonization and infection. The authors find that a 94% improvement in room disinfection led to 35% reduction in patient colonization/infection. AionX 25cleanSURFACESuse a technology registered with the EPA (Reg. No. 91681-1) as an antimicrobial agent that effectively inhibits the growth of bacteria, fungi, algae, mold, and mildew that cause unpleasant odors, discoloration, staining, deterioration or corrosion on surfaces to which it is applied. The EPA has not reviewed or approved,nor does AIONX make, any claims that its technology has efficacy against any particular pathogenic microbes. The use of cleanSURFACES is a supplement to and not acleanSURFACES use a technology is registered with the EPA (Reg. No. 91681-1) as an antimicrobial agent that effectively inhibits the growth of bacteria, fungi, algae, mold, and mildew that cause unpleasant odors, discoloration, staining, deterioration or corrosion on surfaces to which it is applied. The EPA has not reviewed or approved, nor does AIONX make, any claims that its technology has substitute for standard infection control practices.efficacy against any particular pathogenic microbes. The use of cleanSURFACES is a supplement to and not a substitute for standard infection control practices.'